Journal: EMBO Reports
Article Title: Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors
doi: 10.1038/s44319-024-00333-0
Figure Lengend Snippet: Reagents and tools table
Article Snippet: To produce lentiviral media, 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories).
Techniques: Control, Purification, Recombinant, Red Blood Cell Lysis, Activation Assay, Membrane, Staining, Isolation, Plasmid Preparation, DC Protein Assay, Enzyme-linked Immunosorbent Assay, Expressing, shRNA, Amplification, Software, Imaging, Spectrophotometry, Lysis, Protease Inhibitor, Western Blot, SYBR Green Assay, Transfection